This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. These systems are hybrids that allow you to choose between. 더 많은. Fluorescence Microplate Reader Comparison Chart. Weight. High-precision 9 channel system; filter-less with up to 6 wavelengths. Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. Eight filter options provide simplified assay setup. The whole plate can be detect in a single run with ease and flexible. To update the driver. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 0. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. Timothy Bolus, MPM, MBA, Compliance Program Manager. performs within its specifications. View SoftMax Pro GxP. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Exploring misconceptions and understanding the technologies. Summary of Contents for Molecular Devices SpectraMax M2. 1-1. Temperature control and shaking come standard. 76358-624. Step 1: Open the SoftMax Pro IMAP FP protocol. Cellular Imaging Systems. Learn how you can utilize microplate readers to drive landmark discoveries. Select Update Driver. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Find our technical support information along with contact details. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. for SoftMax Pro Software. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 00. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. Plate stacker and robot integration The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within minutes and begingene expression with a user-friendly reader. #4TI-0655) Microplates. 32 was diluted 10-,. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofWe would like to show you a description here but the site won’t allow us. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. SoftMax Pro 7. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The instrument supports the following read modes:. The Spectratest® Abs1 plate, which is used for validationof Molecular Devices monochromator based readers, cannot be used to validate the Emax/Vmax readers. 8000. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. The adapter is required for optimum performance with standard 96-well and 384-. #Flex3) Methods. 6% for the on-board optics (monochromators). For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. With various on-board features and. Molecular Devices' microplate readers. Visible absorbance -. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 314 low lamp level warning. 1. Cell Cytotoxicity assay procedureMolecular Devices. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. Only SoftMax Pro 5 or older versions. We would like to show you a description here but the site won’t allow us. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Step 2: Launch SoftMax Pro Software. Every step is optimized to simplify analysis and reporting to support our microplate readers. It is more inert and reduces compound interference. 1. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. 2, 2022 – Molecular Devices announced today the launch of the ValitaCell IgG titer assay, ValitaTiter, available for global distribution. SpectraMax® Microplate Reader Suite of Products Knowledge Base. These lab workhorses are used in drug discovery. *Purchase is subject to Molecular Devices standard terms and conditions of sale. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. We would like to show you a description here but the site won’t allow us. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. July 17, 2023. Superior optical system includes a xenon flash lamp and features. Versa Max. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Figure 2. Page 17 Chapter 2: Setting Up the Instrument 4. Support. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Recertification service. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Molecular Devices also offers Digital IQ/OQ/PM. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Turn on Plate Reader and wait for it to complete its start-up routine. “Sensitivity is pretty much clutch for luminescence,” says Daly. 1. FlexStation 3Multi-Mode Microplate Reader (Molecular Devices cat. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Multimode microplate readers deliver both performance and economy. SoftMax Pro Software. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. For biochemical assays, the washer removes unbound material and unreacted reagents. The reader measures both flat and round 96-well microplates and. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 1. the iD5 reader’s filter system. Learn moreMolecular Devices has demonstrated the value of a variety of ELISAs for a wide range of research approaches,. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. Skip to main content 美谷分子仪器(上海)有限公司 咨询服务热线:400-821-3787. Ensure data accuracy of your reader. Expanded wavelength coverageAre you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. 0200-6262. Absorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The Beacon Melamine Plate Kit is a competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of melamine in contaminated samples. 1 sec. The primary mode is fluorescence. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular. If it looks fine, then proceed. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ABS Plus can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax ABS and ABS Plus Microplate Readers are a compact and tunable absorbance microplate readers. In . Wait 20 seconds. Next generation SpectraMax® absorbance microplate readers. ©2012 Molecular Devices, LLC | 8/12 | Printed in USA | PN: 0120-1527. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Molecular Devices Software. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Table 1: FLIPR hardware settings for a cell plate basal fluorescence signal test. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Absorbance Plate Reader Comparison Chart. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemPrimary considerations when choosing a microplate reader are throughput and flexibility. Instrument settings can be saved as a protocol file. It also integrates with the. TECHNICAL NOTE. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Tristar 3 Multimode Microplate Reader. 1-2 sec. Recombinant protein expression Nucleocapsid. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. 0 × 107) RLU 0. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. V. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. Plate layout configuration. Plate reader settings used for data acquisition of bacterial growth curve. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Molecular Devices i3x. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 384 deep well plate: packed in sleeves of 6 , 24 per case. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Molecular Devices' microplate readers. 2. To interrupt the kinetic measurement, the. Supersedes 1. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. The instrument settings were identical to those used in Example 1 above. The Versamax Microplate Reader is built around a grating monochromator. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. Monday to Friday 7am - 5pm Pacific Time. 1. you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software Check software compatibility with Windows 10 and locate your software installer. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. SoftMax Pro Software. Eight filter options provide simplified assay setup. #6590-0118) Filter 665/10 (Molecular Devices cat. Endpoint, kinetic, spectrum. Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. AXON Patch-Clamp. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. 306 plate air cal. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Built-in Help function answers your most frequently asked questions. 0 . Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Clone. Microplate Reader, Multimode. Our multi-mode plate readers include absorbance, fluorescence, and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), HTRF®, and AlphaScreen®. Purchase is subject to Molecular Devices standard terms and conditions of sale. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. Select the Driver tab and click Update Driver. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Tests every subsystem of the reader . The new assay will complement Molecular Devices’ workflow solution for cell line development by offering a 10-minute titer assay that can be run on the company’s SpectraMax series of microplate. Microplate Reader Shelf. Data acquisition and analysis. SoftMax® Pro 7. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Plate section without PathCheck applied. Laboratory automation workflows can help. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. Vmax® plate reader specifications. 2 only) SpectraMax M-series Microplate readers. Perform the following steps to install the drivers: Start > Control Panel > System. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. , Sunnyvale, CA 94089, USA, Jin_Liao@moldev. An industry-renowned instrument for monitoring GPCRs and ion channels. 1. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). Complete plate reader solution for all your research needs. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. 9000-0756. , prior to compound addition) from the cells. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Our systems for high-content imaging (HCI) and high-content analysis (HCA) provide flexible scalability making it easy to evolve your system alongside your research. Turn the reader on and secure the USB cable. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Versa. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. One plate carrier adapter is provided with the instrument. Monday to Friday 8am - 5pm GMT. #4TI-0214) UltraVision Plate 96 (Brooks Life. S. #S1205. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. Note that this procedure is only for the SpectraMax L model. Molecular Devices’ AquaMax ® 4000 Microplate Washer aspirates. BMG Labtech - THERMOstar. 1. 1. 304 signal level saturation. Select Start>Control panel>Device Manager. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. FlexIII. Microplate Stacker and Washer. Fibroblasts vs. Endpoint. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. FlexStation® 3 Multi-Mode Microplate Reader. Urban myths of microplate readers. the use of a reader calibrated with primary NIST and NMI standards. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 0 . Plate Formats: 6 to 384-well microplates. Assay flexibility. 1 up to 3600 sec. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. PathCheck sensor corrects optical pathlength & pipetting variation. One plate carrier adapter is provided with the instrument. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. An end-to-end solution for high-content imaging and analysis. 1. coli stock with an OD 600 value of 0. SpectraDrop High-Throughput Screening (HTS) Kit. Get the most out of SoftMax Pro. IMAP assays are based on binding of. Molecular Devices makes. Double-click the unknown device with the yellow warning icon. Extended linear dynamic range. Published Dec 16, 2020 | Updated Dec 16, 2020. The former have been the traditional domain of plate readers and great strides have been. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Click on each for more information. SpectraMax® Microplate Readers and SoftMax® Pro Software. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Step 3. One plate carrier adapter is provided with the instrument. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 0 . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 5. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Important things to consider when purchasing a multimode microplate reader. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. For more sample throughput, the system can be easily integrated into a full roboticThe SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. F3/F5 Multi Mode Microplate Readers. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 2 Software Release Notes. #0200-6182). SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. 0. 2. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The plate reader. Plate stacker and robot integartion. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices Supplier: Molecular Devices Enables data acquisition in endpoint, kinetic, and spectral scan modes at. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for. A. #S1207. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Page 1 of 8 . Supersedes 1. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is preferred for luminescence rather than the core’s multimode readers. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. Use the 1. Every step is optimized to simplify analysis and reporting to support our microplate readers. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. S. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. However, when a specific probe is not available, or it causes off-target effects on the. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. PURPOSE.